MSI – Registration App

In multispectral imaging, acquiring a set of images through different spectral bands is only the beginning — the next essential step is registration, the process of aligning those images so that corresponding points in each band match precisely. This ensures that the spectral information for every pixel refers to the same physical point on the artwork. On this page, we demonstrate how to use our dedicated registration app to align the images captured with the Antonello filter set into a coherent multispectral dataset.

The video lesson walks you through the registration workflow, starting with importing the sequence of images into the app and preparing them for alignment. You will see how the software identifies control points or features across spectral bands, computes the necessary transformations, and applies them to produce an accurately registered stack of images. The user interface is designed to give clear visual feedback on alignment quality, enabling you to refine results where needed.

Accurate registration is foundational for all subsequent MSI analysis steps — whether you are visualizing specific spectral bands, generating false-color composites, or performing classification. Misalignment may lead to incorrect spectral interpretation and visual artifacts, so mastering registration is key to reliable outcomes. By following the video tutorial, you’ll learn how to turn your raw multispectral captures into a well-registered dataset ready for analytical processing.